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Scientist I, Downstream CMC

Boston, MA

The Organization

What's happening at Entrada Therapeutics? Entrada is a clinical-stage biopharmaceutical company aiming to transform the lives of patients by establishing a new class of medicines that engage intracellular targets that have long been considered inaccessible.

Our Endosomal Escape Vehicle (EEV™)-therapeutics are designed to enable the efficient intracellular delivery of a wide range of therapeutics into a variety of organs and tissues, resulting in an improved therapeutic index. Through this proprietary, versatile and modular approach, Entrada is advancing a robust development portfolio of RNA-, antibody- and enzyme-based programs for the potential treatment of neuromuscular, ocular, metabolic and immunological diseases, among others.

Our lead oligonucleotide programs are in development for the potential treatment of people living with Duchenne who are exon 44, 45 and 50 skipping amenable. We have also partnered to develop a clinical-stage program, VX-670, for myotonic dystrophy type 1.

We are a tight-knit team of experts and leaders in both therapeutic development and rare diseases and are excited to grow and attract colleagues who are ready to join a high-energy, dedicated team that likes to get things done and dramatically improve the lives of patients and their families.

The Perfect Addition to Our Team

You are versatile, self-motivated, and looking forward to rolling up your sleeves to contribute to the overarching goals. You enjoy a fast-paced, highly dynamic environment where you’ll learn and grow in the role. You follow up and follow through, connecting problems with solutions efficiently. You are a good communicator and enjoy collaborating with internal and external stakeholders.

The Opportunity

The Scientist I, Downstream CMC will support the company on a critical growth trajectory. Conducting high throughput screens and developing downstream chromatography, including but not limited to Affinity, HIC, IEX, SEC, and MMC. They will perform and lead these activities with minimal supervision. The ideal candidate will interact across different functional groups, including Discovery and Pharmacology. Your ability and desire to thrive in a nimble, fast-paced, results-driven environment will set you up for success.

Responsibilities

  • Perform high-throughput screening of different chromatography resins/columns and identify purification conditions amenable to scale-up for recombinant proteins and protein-conjugates.
  • Execute primary separations (homogenization, solubilization, refold), purification, and formulation.
  • Accountable for designing experimental plans for purifying recombinant proteins and protein conjugates.
  • Execute identified experimental plans independently with minimal supervision, analyze results, and communicate them effectively in group meetings.
  • Coordinate with Upstream, Analytical, and Biologics Discovery groups to complete downstream activities.
  • Develop and generate protocols, procedures, and records to support high throughput screening.
  • Ensure well-organized, clear, and complete records of all experimental activities in laboratory notebooks promptly.
  • Maintain downstream lab equipment and inventory of reagents and consumables in collaboration with other team members.

The Necessities

At Entrada, our passion for science, our devotion to patients and our values drives our behavior:

  • Humanity - We genuinely care about patients and about one another.
  • Tenacity - We are relentless and persistent in the pursuit of developing therapies for patients.
  • Creativity - We are creative problem solvers.
  • Collaboration - We are more than the sum of our parts.
  • Curiosity - We have a growth mindset and push conventional thought and theory.

To thrive on our team, you will need to come with:

  • PhD in Biochemistry, Biochemical Engineering, or related scientific field with 1+ years of experience in purification and characterization of recombinant proteins.
  • Experience purifying recombinant tagless and tagged proteins, including antibodies and enzymes from various hosts, e.g., E. coli, CHO, and Pichia.
  • Skillful in identifying & resolving purification issues or proactively optimizing purification methods. Demonstrated evidence in scientific publications/abstracts/posters is expected.
  • Ability to proactively prioritize and flexibly drive experimental designs.
  • Hands-on experience working with deep well plates (resin screening) and AKTA/NGC FPLC systems to purify recombinant proteins using affinity, HIC, MMC, IEX, and SEC chromatography.
  • Experience with TFF operation to concentrate and diafilter recombinant proteins.
  • Knowledge of biologics downstream process development strategies (including inclusion body solubilization, refolding, column chromatography, conjugation, and formulation) and familiarity with best protein purification practices.
  • Excellent communication skills (oral and written) and ability to work effectively across departments.
  • Growth mindset with the ability to adapt to highly changing priorities.
  • Experience in a fast-growing, dynamic organization; preferably small biotech is preferred.
  • Experience in handling high throughput instruments such as protein maker is preferred.

The Perks

By becoming a team member here at Entrada, you’ll have access to competitive health, dental, and vision coverage, as well as life insurance, and short term and long-term disability insurance. We value work life balance, you'll benefit from discretionary time off, paternity leave, and an excellent 401(k) package. We also offer a generous transportation stipend to commute as you wish. Our location in the vibrant and growing Seaport District is close to all the best that the city of Boston has to offer.

Entrada Therapeutics is an equal opportunity employer. Qualified candidates will receive consideration for employment without regard to race, color, religion, national origin, gender, sexual orientation, gender identity or expression, age, mental or physical disability, and genetic information, marital status, citizenship status, military status, protected veteran status or any other category protected by law.

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Entrada does not accept unsolicited resumes from any source other than directly from candidates. For the protection of all parties involved in the recruiting process, resumes will only be accepted from recruiters/agencies if a signed agreement is in place at the inception of the recruiting effort and authorized for a specified position. Unsolicited resumes sent to Entrada from recruiters/agencies do not constitute any type of relationship between the recruiter/agency and Entrada and do not obligate Entrada to pay fees if we hire from those resumes.

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