Fall 2025 Co-op: Off-target Biology

Company Overview:

Prime Medicine is a leading biotechnology company dedicated to creating and delivering the next generation of gene editing therapies to patients. The Company is leveraging its proprietary Prime Editing platform, a versatile, precise and efficient gene editing technology, to develop a new class of differentiated, one-time, potentially curative genetic therapies. Designed to make only the right edit at the right position within a gene while minimizing unwanted DNA modifications, Prime Editors have the potential to repair almost all types of genetic mutations and work in many different tissues, organs and cell types.  

Prime Medicine is currently progressing a diversified portfolio of eighteen programs initially focused on genetic diseases with a fast, direct path to treating patients or with a high unmet need because they cannot be treated using other gene-editing approaches. Over time, the Company intends to maximize Prime Editing’s therapeutic potential and advance potentially curative therapeutic options to patients for a broad spectrum of diseases. For more information, please visit www.primemedicine.com.

Co-op Assignment Overview

Prime Medicine is seeking a co-op for the Fall 2025 semester with experience in cell culture and molecular biology to join the Off-target team. The candidate will be involved in developing genomic safety packages for pre-clinical programs.

Primary Responsibilities:

• Construction of cell lines to develop controls for off-target assays using Prime Editing and other Gene editing methods
• Analysis of constructed lines and patient material for chromosomal structure using principally Next Generation Sequencing methods
• Validation of these controls and methods for at least one pre-clinical disease 

Qualifications:

• Experience with mammalian cell culture (e.g. transfection, cell handling)
• Experience with Molecular Biology methods (e.g. PCR, DNA quantification, agarose electrophoresis)
• Excited about the potential for gene editing to cure human disease

Preferred Qualifications:

• Exposure and ideally experience with NGS is a plus
• Statistically and analytically savvy with a reasonable knowledge of methods to analyze tabular data
• Experience with gene editing and development of cell lines is an asset

What We Offer:

• A cutting-edge team open to mentorship and networking.
• Full reimbursement for monthly commuting costs including MBTA, Commuter Rail, or Bluebikes, etc.
• Participation in regular seminars with industry experts.
• Full participation in employee activities including learning and development offerings, social activities, etc.

NOTE: We are currently not considering co-op candidates outside of the local Boston/Cambridge area.

Prime Medicine is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, age, sexual orientation, gender identity, disability, veteran status, or any other classification protected by law.