Lab Technician
(ID: 2025-0380)
Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).
Benefits:
- 100% Medical, Dental & Vision Coverage for Employees
- Paid Time Off (Including Holidays)
- Employee Referral Bonus
- 401K Match
- Tuition reimbursement and professional development opportunities
- Flexible Spending Accounts:
- Healthcare (FSA)
- Parking Reimbursement Account (PRK)
- Dependent Care Assistant Program (DCAP)
- Transportation Reimbursement Account (TRN)
Axle is seeking a Lab Technician to join our vibrant team at the National Institutes of Health (NIH) supporting the National Heart, Lung, and Blood Institute (NHLBI) located onsite in Bethesda, MD.
Qualificiations:
- Doctor of Philosophy degree (PhD) in Biology, Molecular and Cell Biology, Immunology, Biochemistry or a related field
- At least three (3) years of related experience in the field(s) of molecular biology, cell biology, vascular biology, immunology or a related field
- Able to independently and proficiently perform cell culture of human cell lines and/or primary human cells is required. Prior experience culturing endothelial cells is preferred
- Ability to independently perform RNA isolation and purification, quantitative RT-PCR, protein isolation (whole cell as well as nuclear and cytoplasmic fractionation), ELISA based assays, Western blotting and light microscopy is required
- Proficiency with flow cytometry and/or confocal microscopy is preferred
- Knowledge of proper laboratory disposal methods and safety procedures
- Experience with Microsoft 365 required. Experience with graphing software (e.g., GraphPad) preferred
- Must be organized, with a strong ability to multi-task, prioritize, and attentive to detail
- Must possess a high level of independent judgment and accuracy
- Ability to make recommendations to effectively resolve issues by using judgment that is consistent with standards, practices, policies, procedures, and regulations or law
- Strong interpersonal skills and an ability to establish and maintain effective working relationships with colleagues and collaborators
- Ability to work both independently and in a team-oriented environment
- Excellent oral and written communications skills including the ability to analyze scientific data and present to groups for peer review
Requirements:
- Independently maintain human cells in culture as well as perform and assist with various experiments and molecular biology techniques and procedures
- Perform RNA and protein isolation from cell lysates
- Perform quantitative RT-PCR and Western blotting
- Perform serum, plasma and PBMC preparation from whole blood for cryopreservation
- Use database for clinical sample tracking
- Maintain electronic laboratory notebook with detailed accounts of reagents and procedures used in experimental work
- In cases of new experimental designs, locate the appropriate references, and then set up, apply, and evaluate the procedure in question
- Collect, organize, analyze, and interpret data. Prepare technical reports, summaries, protocols, standard operating procedures, and inventory updates
- Present data to principal investigator, supervisors, lab members, and collaborators
- Maintain laboratory supply inventories including procurement of laboratory supplies, reagents and equipment
- Oversee general lab maintenance and organization including equipment maintenance
- Troubleshoot problems with equipment or experimental techniques and develop methods to resolve these difficulties under the direction of the task leader
- Maintain laboratory safety and update records of safety trainings
- Operate and communicate effectively in a group, team-oriented working environment
- Comply with institution’s rules and regulations, such as laboratory safety and waste disposal methods, institutional review board protocols, environmental health and safety
The diversity of Axle’s employees is a tremendous asset. We are firmly committed to providing equal opportunity in all aspects of employment and will not tolerate any illegal discrimination or harassment-based age, race, gender, religion, national origin, disability, marital status, covered veteran status, sexual orientation, status with respect to public assistance, and other characteristics protected under state, federal, or local law and to deter those who aid, abet, or induce discrimination or coerce others to discriminate.
Accessibility: If you need an accommodation as part of the employment process please contact: careers@axleinfo.com
Disclaimer: The above description is meant to illustrate the general nature of work and level of effort being performed by individual’s assigned to this position or job description. This is not restricted as a complete list of all skills, responsibilities, duties, and/or assignments required. Individuals may be required to perform duties outside of their position, job description or responsibilities as needed.
This role has a market-competitive salary with an anticipated base compensation range listed below. Actual salaries will vary depending on a candidate’s experience, qualifications, skills, and location.
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Salary Range
$90,000 - $100,000 USD
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